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Ion-selective membrane with antimicrobial effect
Gablech, Evelína ; Farka,, Zdeněk (oponent) ; Soukupová,, Jan (oponent) ; Drbohlavová, Jana (vedoucí práce)
This work deals with improvement of antimicrobial properties of commercial polymer membranes used in dairy industry for filtration of milk products such as whey. These membranes suffer from contamination caused by microorganisms present in milk during the filtration process. This biological contamination affects the life-time of the membrane and also filtration process itself. Thus, these membranes were modified with plasma treatment to incorporate amine functional groups for subsequent immobilization of silver and selenium nanoparticles to prevent the membranes from creation of biofilm of microorganisms. Antimicrobial activity of both types of nanoparticles and membranes with immobilized nanoparticles was determined against cell strains Staphylococcus aureus and Escherichia coli.
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The influence of modified TiO2 nanotubes on biointerfacial interaction
Bílek, Ondřej ; Kočí,, Kamila (oponent) ; Hlaváček,, Antonín (oponent) ; Fohlerová, Zdenka (vedoucí práce)
Over the past few years, titanium dioxide nanotubes have become increasingly important in biomedicine. As a biocompatible nanostructured surface, it finds the potential for its application especially in the field of implant applications. The theoretical part of this thesis, therefore, deals with options for TiO2 nanotube preparation, and their use as a biomedical material. The review of conducted studies includes bare, surface-modified and doped nanotubes. The experimental part discusses titanium dioxide nanotubes, which are prepared from titanium by a one-step anodic oxidation method in an organic electrolyte. Titanium-sputter-deposited silicon wafers and titanium foils are used as a substrate. The initially amorphous nanotubes are annealed, and subsequently modified with selenium and silver nanoparticles. The prepared structures are studied in terms of surface properties, and biological interactions with selected tissue cells (MG-63, NIH-3T3) and bacteria (E. coli, P. aeruginosa S. aureus). The final part of experimental work is dedicated to brief comparison of selenium and silver nanoparticle modified TiO2 nanotubes. The main aim of this thesis is to extend the knowledge regarding the bio-interface formed by adherent cell lines, bacterial cells, and selenium/silver modified TiO2 nanotubes.
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Preparation and characterisation of encapsulated biogenic nanoparticles for medical application
Poláková, Veronika ; Vojtová, Lucy (oponent) ; Brtníková, Jana (vedoucí práce)
The aim of this bachelor thesis is a preparation and polymeric encapsulation of antibacterial biogenic nanoparticles in order to enhance their stability, reduce possible cytotoxicity while maintaining antibacterial activity. The theoretical part contains an overview of regenerative medicine, commonly used nanostructures in regenerative medicine, their properties, and methods of encapsulation. The experimental work especially focuses on selenium nanoparticles synthesis using different methods with specific protecting agents followed by encapsulation via nature-inspired polymer. The used encapsulation methods are based on self-assembly polymerization and coating of selected natural polymeric adhesive. The chemical and physical properties of pure and encapsulated selenium nanoparticles, such as their concentration and morphology (size and shape) were studied using Fourier transformed infrared spectrophotometry and scanning transmission electron microscopy. It was found that different used method provides nanoparticles with different size, shape and stability. As a main result, an optimized method of selenium particles synthesis, stabilization and encapsulation was developed and described. Nanoparticles, synthesized using this method, are spherical with size ranging from 10.5 to 101 nm. The sizes of most of the synthesized nanoparticles lay within 10.5 to 40 nm interval. When encapsulated, their sizes increase and are ranging from 74.5 to 571.5 nm.
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Ion-selective membrane with antimicrobial effect
Gablech, Evelína ; Farka,, Zdeněk (oponent) ; Soukupová,, Jan (oponent) ; Drbohlavová, Jana (vedoucí práce)
This work deals with improvement of antimicrobial properties of commercial polymer membranes used in dairy industry for filtration of milk products such as whey. These membranes suffer from contamination caused by microorganisms present in milk during the filtration process. This biological contamination affects the life-time of the membrane and also filtration process itself. Thus, these membranes were modified with plasma treatment to incorporate amine functional groups for subsequent immobilization of silver and selenium nanoparticles to prevent the membranes from creation of biofilm of microorganisms. Antimicrobial activity of both types of nanoparticles and membranes with immobilized nanoparticles was determined against cell strains Staphylococcus aureus and Escherichia coli.
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The influence of modified TiO2 nanotubes on biointerfacial interaction
Bílek, Ondřej ; Kočí,, Kamila (oponent) ; Hlaváček,, Antonín (oponent) ; Fohlerová, Zdenka (vedoucí práce)
Over the past few years, titanium dioxide nanotubes have become increasingly important in biomedicine. As a biocompatible nanostructured surface, it finds the potential for its application especially in the field of implant applications. The theoretical part of this thesis, therefore, deals with options for TiO2 nanotube preparation, and their use as a biomedical material. The review of conducted studies includes bare, surface-modified and doped nanotubes. The experimental part discusses titanium dioxide nanotubes, which are prepared from titanium by a one-step anodic oxidation method in an organic electrolyte. Titanium-sputter-deposited silicon wafers and titanium foils are used as a substrate. The initially amorphous nanotubes are annealed, and subsequently modified with selenium and silver nanoparticles. The prepared structures are studied in terms of surface properties, and biological interactions with selected tissue cells (MG-63, NIH-3T3) and bacteria (E. coli, P. aeruginosa S. aureus). The final part of experimental work is dedicated to brief comparison of selenium and silver nanoparticle modified TiO2 nanotubes. The main aim of this thesis is to extend the knowledge regarding the bio-interface formed by adherent cell lines, bacterial cells, and selenium/silver modified TiO2 nanotubes.
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